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Induction of Resistance to Toxoplasma Gondii in Human Macrophages by Soluble Lymphocyte Products¹

Division of Allergy, Immunology and Infectious Diseases, Palo Alto Medical Research Foundation, Palo Alto, California 94301 and Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, California 94305

Abstract

Autoradiography and light microscopy were used to study the effects of lymphocyte culture supernatants, prepared under a variety of conditions, on the course of intracellular Toxoplasma gondii infection in human monocyte-derived macrophages in vitro. Supernatants prepared by incubating lymphocytes of dye test- (DT) positive subjects with T. gondii lysate antigen (TLA), lymphocytes of DT/negative subjects with streptokinase-streptodornase (SK-SD), or both populations of lymphocytes with concanavalin A (Con A) were capable of activating macrophages to inhibit or kill intracellular T. gondii. Supernatants prepared with the homologous antigen (TLA) to the target organism appeared more active in conferring resistance to infection with T. gondii on macrophages than those prepared with a heterologous antigen (SK-SD) or mitogen (Con A). The number of lymphocytes was critical in preparing active supernatants. These results suggest that soluble lymphocyte mediators can activate human macrophages in vitro to inhibit or kill T. gondii.

Footnotes

¹ This work was supported by Grant AI 04717 from the National Institutes of Health and Training Grant AI 00260 from the National Institutes of Health. The opinions or assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large.

² Special Fellow, Bureau of Medicine and Surgery, U.S. Navy. Present address: Division of Infectious Diseases, Department of Medicine, National Naval Medical Center, Bethesda, Maryland 20014.

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				J. Y. Channon, R. M. Seguin, and L. H. Kasper Differential Infectivity and Division of Toxoplasma gondii in Human Peripheral Blood Leukocytes Infect. Immun., August 1, 2000; 68(8): 4822 - 4826. [Abstract] [Full Text] [PDF]