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Structural Studies on Induced Antibodies with Defined Idiotypic Specificities

III. N-Terminal Amino Acid Sequence of the Heavy and Light Chains of Mouse anti-Streptococcal Antibodies—A5A, S8, and S117¹

From the Department of Microbiology, The University of Texas Health Science Center, Southwestern Medical School, Dallas, Texas 75235, and the Institute for Genetics, University of Cologne, West Germany

Abstract

The murine V_H genetic map consists of two different subloci of V_H genes. Seven idiotypes have been allocated to these two different subloci, one of which contains genes coding for the A5A idiotype, while the second contains the genes encoding for six other idiotypic markers. Previous studies from this laboratory have shown that anti-Ars antibodies are best classified in the murine V_HII subgroup. Studies from other laboratories indicate that the J558 idiotype could be classified in a similar fashion. The sequence of the T15 myeloma protein V_H region is most characteristic of the murine V_HIII subgroup. These studies were undertaken in order to study the V_H subgroup of two additional murine V_H markers. Since one of these markers has been allocated to a separate sublocus, comparisons can be made for the first time between the "framework" residues (V_H subgroup) and the murine genetic map. The data indicate that within a single presently defined sublocus proteins exist which contain remarkably different "framework" residues.

Footnotes

¹ This work was supported in part by Grant BMS 74-01441 from the National Science Foundation, AI 12127 and AI 12796 from the National Institutes of Health, and Sonderforschungsbereich 74 from the Deutsche Forschungsgesellschaft.