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Blocking of MLC Stimulation by Anti-Ia Sera: Studies with the Virus Plaque Assay¹

From the Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461 and the Department of Genetics, Washington University, St. Louis, Missouri 63110

Abstract

The development of congenic mouse strains identical at the H-2K and H-2D loci but differing by I-region associated (Ia) determinants has permitted an association to be established between Ia determinants and stimulation in mixed lymphocyte culture reactions (MLR). The present experiments were undertaken to establish whether the Ir-coded control of MLR operated at the level of recognition or of stimulation. Reciprocal MLR were established between A.TH and A.TL mouse spleen cells in the presence or absence of anti-Ia sera directed either at determinants of the stimulating or responding cells. The number of T cells responding was assessed by the virus plaque assay.

Anti-Ia sera directed against the responding cells were no more inhibitory of the MLR than normal mouse serum. In contrast, anti-Ia sera directed against determinants of the mitomycin-treated stimulating cells markedly inhibited activation of T cells in the MLR.

Footnotes

¹ This work was supported by United States Public Health Service, Grants AI 09807 and AI 10702.

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				B. R. Bloom, A. Senik, G. Stoner, G. Ju, M. Nowakowski, S. Kano, and L. Jimenez Studies on the Interactions between Viruses and Lymphocytes Cold Spring Harb Symp Quant Biol, January 1, 1977; 41(0): 73 - 83. [Abstract] [PDF]