HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Czarnetzki, B. M. Articles by Lichtenstein, L. M. Search for Related Content PubMed Articles by Czarnetzki, B. M. Articles by Lichtenstein, L. M.

Eosinophil Chemotactic Factor (ECF)

I. Release from Polymorphonuclear Leukocytes by the Calcium Ionophore A23187¹

From The Johns Hopkins University School of Medicine at The Good Samaritan Hospital, Clinical Immunology Division, 5601 Loch Raven Boulevard, Baltimore, Md. 21239

Abstract

Eosinophil chemotactic factor (ECF), previously thought to be primarily associated with human basophils and mast cells, could be released from human neutrophils (PMN) and eosinophils but not lymphocytes by the calcium ionophore A23187. Release of ECF from PMN was time and dose-dependent. Like antigen-induced, basophil-derived ECF, PMN-derived ECF had a high selectivity for eosinophils as determined by differential counts of migrating cells and by deactivation studies. Chromatographic analysis of PMN-derived ECF on Sephadex G-25 showed an elution pattern very similar to that of basophil-derived ECF. With rat peritoneal cells, it was possible to show that mast cells as well as mast cell-depleted cell preparations could be induced to release an ECF that appears to be similar or identical to human ECF. These findings suggest that ECF may play a role in inflammatory processes involving cells other than basophils and mast cells.

Footnotes

¹ This work was supported by Grants 07290 and 08270 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland. This is publication No. 000, O'Neill Research Laboratories, The Good Samaritan Hospital.

² Recipient of the Stetler Research Fund for Women Physicians.

This article has been cited by other articles:

				F. H. Falcone, A. G. Rossi, R. Sharkey, A. P. Brown, D. I. Pritchard, and R. M. Maizels Ascaris suum-Derived Products Induce Human Neutrophil Activation via a G Protein-Coupled Receptor That Interacts with the Interleukin-8 Receptor Pathway Infect. Immun., June 1, 2001; 69(6): 4007 - 4018. [Abstract] [Full Text] [PDF]