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Studies on the Immune Response to Fixed Antigens. Preferential Induction of Helper Function with Heavily Trinitrophenylated Sheep Erythrocytes, and Glutaraldehyde-Treated Sheep Erythrocytes¹

From the Lautenberg Center for General and Tumor Immunology, The Hebrew University, Hadassah Medical School, Jerusalem

Abstract

Mice primed with heavily trinitrophenylated sheep red cells (TNP₁₂₈SRC) or glutaraldehyde-treated sheep red cells (G-SRC) developed an early helper function mediated by thymus-derived cells. Such mice were able to produce high secondary responses to both hapten and carrier after challenge 2 days after priming, with lightly trinitrophenylated SRC (TNP _0.14SRC). However, the primary response of the TNP ₁₂₈SRC or G-SRC-primed mice were very low to undetectable, and their secondary responses were also low when the challenge antigen was administered 4 days after priming or later. Inhibitory humoral factor(s) which were induced in the primed animals appeared responsible for the decreased capacity of primed mice to mount a secondary response when challenged later than 2 days after priming. Transfer of spleen cells from TNP ₁₂₈SRC-primed mice to sublethally irradiated recipients circumvents their exposure to inhibitory humoral factor(s) present in intact animals allowing them to react with challenge antigen. Enriched populations of T cells, but not B cells, were able to transfer this early immunologic memory to irradiated recipients. The theoretical and practical implications of these results are discussed.

Footnotes

¹ This work was supported by research grants from the Leukemia Research Foundation, Inc., Concern Foundation, Inc., The Lautenberg Endowment Fund, and Mr. and Mrs. Laurence Tisch.