HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Trivers, G. Articles by Leonard, E. J. Search for Related Content PubMed Articles by Trivers, G. Articles by Leonard, E. J.

Mouse Lymphotoxin

From the Tumor Antigen Section, Biology Branch, National Cancer Institute, Bethesda, Maryland, 20014, and the Department of Biology, Catholic University, Washington, District of Columbia

Abstract

The addition of PHA to C3H mouse spleen cells in tissue culture led to the production of lymphotoxin (LT). Cytotoxicity was assayed by addition of the culture fluids to syngeneic target cells labeled with tritiated thymidine; after an incubation period of 72 hr the amount of radioactivity released into the supernatant was measured. The LT activity in unfractionated culture fluids survived lyophilization, remained unchanged for many weeks at 4°C, and progressively decreased on heating at 56°C for periods from 1 to 9 hr. Based on the G-200 Sephadex distribution coefficients for several preparations, the m.w. of mouse lymphotoxin was about 41,000 daltons. Lymphotoxin from three different spleen cell production runs was recovered from isoelectric focusing columns in sharply focused peaks, the pH of which ranged from 4.4 to 4.8.

Footnotes

¹ This work was performed in partial fulfillment of the requirements for Ph.D. in the Department of Biology, Catholic University, Washington, District of Columbia.

² Address reprint requests to Dr. Leonard, Bldg. 37, Room 2B21, National Cancer Institute, Bethesda, Maryland 20014.