HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lint, T. F. Articles by Gewurz, H. Search for Related Content PubMed Articles by Lint, T. F. Articles by Gewurz, H.

Bystander Hemolysis by Complement Activation in the Fluid Phase

Department of Immunology, Rush Medical College, Chicago, Ill.

Abstract

Activation of guinea pig (GP) C by zymosan (Z), antigen-antibody complexes (Ag-Ab), aggregated human -globulin or agarose resulted in hemolysis of bystander GPE, which was optimal at 17°C. Lysis was greatly potentiated by polycations such as poly-L-lysine (PLL) and leukocyte cationic proteins, which counteracted serum
-inhibitors (
-INH), an activity displayed by a group of serum substances which include lipoproteins, to favor expression of the hemolytic potential of
formed in solution. Bystander cell hemolysis also was induced by C activation at 17°C of suitably absorbed human serum using Z or Ag-Ab. This was dependent on serum, cell, and activator concentrations, and was not observed with heat-inactivated sera; it occurred in EGTA when Mg⁺⁺ (6mM for optimum lysis) was added.
-INH also inhibited Z-induced lysis in human serum and PLL could be shown to potentiate this lysis provided it was added subsequent to formation of the C5 convertase.