HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Brade, V. Articles by Hadding, U. Search for Related Content PubMed Articles by Brade, V. Articles by Hadding, U.

The Role of Factor D for the Interaction of Zymosan with Components of the Properdin System: Studies with D-Depleted Guinea Pig Serum

Institut für Medizinische Mikrobiologie der Universität Mainz, Germany

Abstract

Serum was depleted of factor D by gel filtration and immunoabsorption with unsolubilized anti-D IgG. Substitution of this reagent (RD) with purified was found to fully reconstitute its function. In the following experiments the reaction of zymosan (Z) with RD was investigated; therefore these studies are concerned with the role of Factor D for the interaction of Z with components of the properdin system.

RD was incubated for 30 min at 0°C, or for 30 min at 37°C, with Z (10 mg/ml) in the presence of EGTA. After centrifugation both supernatants, as well as the corresponding Z particles, were analyzed. Both supernatants were found to contain identical amounts of B, whereas C3 was slightly reduced in the 37°C supernatant. After addition of Z and of purified , both supernatants supported the formation of the C3-cleaving ZX-complex with the same efficiency as untreated RD. Thus, incubation of Z with RD was only followed by a slight loss of C3; no other factors of the properdin system appeared to be reduced in their activity.