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Evidence for a T* Intermediate in the Antibody-Complement Mediated Killing of Tumor Cells

National Cancer Institute, Bethesda, Md. 20014

Abstract

The ascitic form of a chemically induced guinea pig hepatoma cell, line-1, can be killed with antibody and guinea pig complement (GPC). The rate and extent of killing, in the presence of excess complement, is dependent on the concentration of antibody used to sensitize the cells. By using a limiting dilution of antibody, it was possible to demonstrate a temporal difference between the fixation of complement and death of the cell. This suggested that in addition to the fixation of complement there was a further intrinsic transformation step leading to cell lysis and that this step was rate limiting. Experiments were performed to determine if, in this tumor system, an intermediate equivalent to E* in the sheep red cell system was formed.

The postulated T* was prepared by incubating line-1 cells with a limiting dilution of anti-Forssman antibody and GPC for 5 min at 37°C. At this time the cells were washed free of unbound complement.