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Studies on the Antigenic Determinants of the Thy-1.2 Alloantigen as Expressed by the Murine Lymphoblastoid Line S-49.1 TB·2·3^1,2,

From the Department of Microbiology, University of Alabama Medical Center, Birmingham, Alabama 35294

Abstract

Sialic acid has been shown to be part of the antigenic determinant of the Thy-1.2 alloantigen as expressed by the murine cell line S-49.1 TB·2·3 (S-49). This conclusion is based on the loss of cytotoxic inhibitory activity by the action of neuraminidase on the Thy-1.2 alloantigen. Also, sialic acid has the ability to inhibit the cytotoxic assay of AKR anti-C3H Thy-1.2 serum for S-49 cells. Further evidence for the protein nature of the Thy-1.2 alloantigen is apparent by trypsin digestion. Possibly the Thy-1.2 alloantigen as expressed on S-49 cells is a glycoprotein.

Footnotes

¹ This work was supported by Grant AI 11970 and AM 03555 from the National Institutes of Health and Contract N01-CB 44009 from the National Cancer Institute.

² Cells used in this study were grown in the Cell Culture Facility supported by Grant BMS74-12080A01 from the Human Cell Biology Section of the National Science Foundation.

³ Recipient of an Institutional Research Fellowship, Award T22GM 00130, from the National Institute of General Medical Sciences.