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Anti-Immunoglobulin Stimulation of Murine Lymphocytes¹

I. Age Dependency of the Proliferative Response

From the Division of Clinical Immunology, Departments of Medicine and Microbiology, University of Colorado Medical School, Denver, Colorado 80220

Abstract

The in vitro proliferative response of normal mouse spleen cells to anti-immunoglobulin (Anti-Ig) reagents was found to be an age-associated phenomenon. The response usually appears in mice when they reach 5 to 7 months of age and is rarely seen in younger animals. Anti-Ig induced proliferation was observed by using two different antisera—one polyvalent, prepared against mouse antibody-antigen complexes and one prepared against mouse IgM myeloma. Both antisera were shown to be specific for B cells by cytotoxicity and immunofluorescent staining. Glassbead separation of spleen cells showed that the anti-Ig-induced proliferation was a B cell response.

Footnotes

¹ This work was supported in part by National Institutes of Health Grant HDO 7779 and by the Colorado Multiple Sclerosis Society.

² Fellow of the Colorado Multiple Sclerosis Society.

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