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Specific Suppression of the Immune Response by Hgg Tolerant Spleen Cells

I. Parameters Affecting the Level of Suppression¹

From Scripps Clinic and Research Foundation, Department of Immunopathology, 476 Prospect Street, La Jolla, California 92037

Abstract

After adoptive transfer, the spleen cells from mice made tolerant to human -globulin (HGG) specifically suppress the immune response of normal spleen cells. However, this suppressive activity in the spleen cells of tolerant mice is only present for a brief period after treatment with tolerogen. Spleen cells from animals injected 10 days earlier with tolerogen reduce the immune response of an equal number of normal spleen cells by 75%. Spleen cells from mice made tolerant 40 days previously are, however, no longer suppressive, even though they remain completely unresponsive. These data suggest that active suppression of antigenreactive cells is not the mechanism responsible for maintaining tolerance to HGG, but rather is only transiently associated with the tolerant state. Further evidence in favor of the separation of the tolerant state from suppressive activity is that complete suppression of the normal spleen cell response requires either a high ratio of tolerant to immune competent cells or a delay in the antigenic challenge of the reconstituted recipients. By contrast, such manipulations are not required to demonstrate the complete unresponsiveness of the tolerant cells after adoptive transfer.

Footnotes

¹ This is Publication No. 1073 from the Department of Immunopathology, Scripps Clinic and Research Foundation, La Jolla, California. The work was supported by United States Public Health Service Grant AI-07007, Atomic Energy Administration Contract E (04-3)-410 and American Cancer Society Grant IM-42F.

² Recipient of a National Institutes of Health Fellowship Award No. AI-01023.

³ Recipient of a National Institutes of Health Fellowship Award No. AI-05012.

⁴ Recipient of United States Public Health Service Research Career Award 5-K6-GM-6936.

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