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The Formation of Septate-Like Junctional Complexes between Lymphoid Cells in Vitro¹

From the Departments of Medicine and Pathology, Medical University of South Carolina, 80 Barre Street, Charleston, South Carolina 29401 and Department of Pathology, Harvard Medical School, 25 Shattuck Street, Boston, Massachusetts 02115

Abstract

During the initial 48 hr of incubation in immunized Mishell-Dutton spleen cell cultures, most lymphocytes exist as single cells or an occasional pair of cells (doublets). Ultrastructural examination of the area between those cells forming doublets revealed a septate-like junctional complex occurring over large portions of the plasmalemma. This junctional zone persisted in areas where extensive cytoplasmic interdigitation among cell processes was evident. Approximately 1% of the lymphocytes isolated from the top fraction of a discontinuous fetal calf serum gradient were involved in formation of doublets. The majority of these doublets showed evidence of junctional interaction. Moreover, this septate-like zone of adhesion was seen using several different fixatives and stains and remained intact after osmotic cell disruption. The junction was not demonstrable by using freeze-fracture techniques and therefore must be limited to interactions within the surface of the cells.

Footnotes

¹ This investigation was supported by Grants HL-09125, AI-09897 and Training Grant GM-02135 from the National Institutes of Health, United States Public Health Service. Dr. Carl W. Pierce is the recipient of Research Career Development Award 5K04-AI-70173 from the National Institutes of Health, United States Public Health Service.

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