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The Journal of Immunology, 1976, 116: 1507-1511.
Copyright © 1976 by The American Association of Immunologists, Inc.
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In Vivo Protection Against Syngeneic Gross Virus-Induced Lymphoma in Rats: Comparison with in Vitro Studies of Cell-Mediated Immunity

Moshe Glaser1, David H. Lavrin and Ronald B. Herberman

From the Laboratory of Immunodiagnosis, National Cancer Institute, Bethesda, Maryland 20014, and Litton-Bionetics, In., 5516 Nicholson Lane, Kensington, Maryland 20795

Abstract

Spleen cells at various times after inoculation of W/Fu rats with a syngeneic Gross virus-induced lymphoma, (C58NT)D, were tested for their in vivo activity in adoptive transfer experiments and for their in vitro reactivity in a 4-hr 51Cr release cytotoxicity assay and in a mixed lymphocyte-tumor cell interaction assay. In adoptive transfer, the best protection against tumor growth was observed with immune spleen cells taken at 30 days after tumor cell inoculation (the peak of reactivity in the mixed lymphocytetumor cell interaction assay) whereas cells taken at 10 days (the peak reactivity in the 51Cr release cytotoxicity assay) gave only partial protection. The protection detected in the adoptive transfer experiments was specific for (C58NT)D associated antigens, and this correlated well with the specificity observed in the in vitro cell-mediated immunity assays. T cells, but not complement receptor-bearing cells or macrophages, were essential for the protection against tumor growth in vivo, and also for the in vitro reactivity in the 51Cr release cytotoxicity and the mixed lymphocytetumor cell interaction assays.

Footnotes

1 Address reprint requests to: Moshe Glaser, Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland 20014.






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