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Macrophage Requirements of CR^- and CR⁺ B Lymphocytes for Antibody Production in Vitro¹

From the Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021

Abstract

A Sephadex G-10 column coated with antigen-antibody complexes and complement retains complement receptor-bearing (CR⁺) mouse spleen cells. The effluent is rich in thymus-derived cells (T cells), and contains bone marrow-derived cells (B cells) which carry surface immunoglobulin (Ig), Ir-associated antigen (Ia), and Fc receptors, but no complement receptors (CR^-). Although both unfractionated and CR^- B cell populations are capable of producing antibody to red cell antigens, they differ in their requirements for the initiation of the response. Unfractionated B cells cooperate with primed as well as unprimed helper T cells; macrophages are required for this cooperation but can be replaced by 2-mercaptoethanol. CR^- B cells cooperate with primed but not with unprimed T cells provided macrophages are added to cultures. After addition of culture supernatant from BCG-activated macrophages CR^- B cells cooperate with both unprimed and primed T helper cells.

Footnotes

¹ This work was supported by National Cancer Institute Grants CA 08748, CA 16899 and CA 17673-01.