HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Harwell, L. Articles by Marrack, P. Search for Related Content PubMed Articles by Harwell, L. Articles by Marrack, P.

Antigen-Specific and Nonspecific Mediators of T Cell/B Cell Cooperation

III. Characterization of the Nonspecific Mediator(s) from Different Sources¹

From the Department of Microbiology, Division of Immunology, and the University of Rochester Cancer Center, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642

Abstract

T cell-containing lymphoid populations produce a nonantigen-specific mediator(s) (NSM) which can replace T cell helper function in vitro in the response of B cells to sheep red blood cells (SRBC), but not to the hapten-protein conjugate, trinitrophenyl-keyhole limpet hemocyanin, (TNP-KLH). NSM produced under three conditions: 1) stimulation of KLH-primed cells with KLH; 2) allogeneic stimulation of normal spleen cells; and 3) stimulation of normal spleen cells with Con A (but not PHA) are indistinguishable on the basis of their biologic activity and m.w., estimated as 30 to 40,000 daltons by G-200 chromatography. Production of NSM is dependent on the presence of T cells. The action of NSM on B cells responding to SRBC in the presence of 2-mercaptoethanol is unaffected by severe macrophage depletion. Extensive absorption of NSM with SRBC failed to remove its activity, confirming its nonantigen-specific nature.

Footnotes

¹ This work was supported by the National Institutes of Health Research Grants CA-11198 and AI-11558, and the American Cancer Society Research Grant IM-49.

This article has been cited by other articles:

				D. H. Katz The Role of the Histocompatibility Gene Complex in Lymphocyte Differentiation Cold Spring Harb Symp Quant Biol, January 1, 1977; 41(0): 611 - 624. [Abstract] [PDF]