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Antigen-Specific and Nonspecific Mediators of T Cell/B Cell Cooperation

II. Two Helper T Cells Distinguished by Their Antigen Sensitivities¹

From the Department of Microbiology, Division of Immunology and the University of Rochester Cancer Center, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642

Abstract

Further evidence is presented for two types of helper T cells in the mouse specific for the protein antigen, keyhole limpet hemocyanin (KLH). The first cell helps B cells respond to the trinitrophenyl hapten (TNP) coupled to KLH, is primed by relatively high doses of antigen in vivo, and yet the effector cell is stimulated by very low doses of antigen in vitro. The second cell helps B cells respond to a non-cross-reacting antigen, sheep red blood cells, presumably via production of a nonspecific factor. This cell is primed by relatively low doses of antigen in vivo, but the effector cell requires relatively high doses of antigen in vitro. Thus, the two T cell types are differently sensitive to antigen dose, both in priming and challenge. The properties of T cells responding to KLH by proliferation in vitro were also studied. These cells showed the same antigen-sensitivity in vitro, as cells producing nonspecific B cell-stimulating factors.

Footnotes

¹ This work was supported by United States Public Health Service Research Grants AI-11558 and CA-11198 and American Cancer Society Grant IM-49.