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The Journal of Immunology, 1976, 116: 1224-1227.
Copyright © 1976 by The American Association of Immunologists, Inc.
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Preparation and Effects of an Anti-B Cell Serum1

Terutaka Kakiuchi, Hideo Nariuchi2 and Noboru Tamura

From the Department of Allergology, Institute of Medical Science, University of Tokyo, Minatoku 108 Tokyo and the Virology Division, National Cancer Center Research Institute, 5-1, Tsukiji, Chuo-ku, 104 Tokyo, Japan

Abstract

An heterologous antiserum specific for bone marrow-derived cells (B cells) was prepared by immunizing rabbits with lymph node cells from nude mice. After absorption with mouse red blood and thymus cells, the antiserum killed a population of cells from various lymphoid organs and the cytotoxic effects were inversely related to those of anti-{vartheta} antibody.

When bone marrow or spleen cells were treated with the antiserum and guinea pig complement before transfer into irradiated mice, the number of plaque-forming cells was greatly reduced in the spleen of the recipient. Pretreatment of thymus cells with the antiserum in a similar way resulted in no inhibition of hemolytic plaque. When spleen cells from mice previously immunized with sheep red blood cells were treated with the antiserum and complement, the formation of hemolytic plaque was not affected.

These findings indicated that the antiserum was specific for B cells and that the number of antigenic determinants on B cells to which the antiserum reacted decreases during differentiation into antibody-forming cells.

Footnotes

1 This work was supported in part by Grants for Scientific Research from the Ministry of Education and the Ministry of Public Welfare of Japan.

2 Present address: Laboratory of Cellular and Comparative Physiology, Gerontology Research Center, Baltimore City Hospitals, Baltimore, Maryland 21224






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