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Characterization of T and B Antigen-Binding Cells for -Galactosidase

II. T Antigen-Binding Cells^1,2,

From the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

Abstract

The results reported in this paper suggest that the specific Z-binding cells of the normal mouse include a large portion of T lymphocytes. Depletion of T cells with anti- serum and cortisone indicates that the majority of the ZBC of the thymus, which occur at frequencies of about 150/10⁶, are indeed T cells. Similar treatment of spleen cells suggests that approximately half the binding cells in that organ are contributed by the T lymphocyte population. T- and B-enriched populations obtained from the spleen by using differential adherence to nylon wool contained equal numbers of ZBC and bound equivalent amounts of the antigen. Hence, there appears to be a high frequency of T lymphocytes that can be shown to bind -galactosidase specifically in both the thymus and spleen of normal mice.

Footnotes

¹ This work is based on a thesis submitted by S. L. Swain in partial fulfillment of the requirements for the degree of Doctor of Philosophy, Harvard University. The work was supported in part by Grant AI 05691 from the National Institutes of Health and (S.S.) Training Grant 5T-01-AI 00387.

² Presented in part at the annual meeting of the Federated Societies for Experimental Biology, April 1974 (Fed. Proc. 33: 802).

³ Present address: Dept. of Biology, U.C.S.D., La Jolla, California.

⁴ Present address: Dept. of Pathobiology, School of Public Health, Teheran University, Teheran, Iran.

⁵ Career Investigator, American Heart Association. Please send reprint requests to him.