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A Phosphorycholine Idiotype Related to TEPC 15 in Mice Infected with Ascaris Suum¹

From the Department of Immunology and Medical Microbiology, and the Department of Pathology, College of Medicine, University of Florida, Gainesville, Florida 32610

Abstract

A serum component, binding antigens having phosphorylcholine (PC) determinants were induced in several strains of mice by infection with Ascaris suum. This component was isolated and demonstrated to be an IgM () anti-PC antibody having idiotypic determinants in common with the IgA PC-binding myeloma protein TEPC 15. Rabbit anti-idiotypic antisera prepared with this component had idiotypic specificity for TEPC 15 and cross-idiotypic recognition of MOPC 167 and McPC 603, all IgA PC-binding myeloma proteins. The antisera also recognized determinants not present on TEPC 15.

IgM and idiotype levels were quantitated by radial immunodiffusion and PC-specific antibody measured by hemagglutination (HA) with sheep erythrocytes coated with pneumococcal-C-polysaccharide. Mean IgM levels ranged from 2.5 to 8.7 mg/ml, idiotype from 0.54 to 5.3 mg/ml; and HA titers from 1:512 to 1:130,000 in different mouse strains. The high PC-specific antibody response was duplicated by immunization with dead ascaris larvae or by infection with two other nematode species.

Footnotes

¹ This work was supported in part by Public Health Service Grants AI-03212 and AI-05345 from the National Institute of Allergy and Infectious Diseases, and Training Grant 5TI AI-0128.

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