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Radioimmunoassay for Detection of Antigen and Antibodies to Newcastle Disease Virus¹

From the Chanock Center for Virology, Hebrew University-Hadassah Medical School, Jerusalem, Israel

Abstract

When Newcastle disease virus (NDV) is treated with NP-40 and ether a membrane fraction of 150.000 m.w. is obtained. This fraction which is composed of two polypeptides with m.w. of 56.000 and 76.000 was used in a radioimmunoassay (RIA). The assay was developed for both antigen and antibody and was found to be reproducible, specific, and highly sensitive. Titers of 1:51,200 were determined by RIA as compared to 1:4 by agar gel diffusion and 1:200 by hemaglutination inhibition (HI). As little as 5 ng of viral protein were detected by RIA inhibition technique.

Labeled antigen could be stored in the presence of serum, KCI and Triton X-100 at -20°C for as long as 6 weeks and retained similar reactivity as fresh reagent.

Footnotes

¹ This work was supported by the Chanock Foundation.