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Eosinophils and Immune Mechanisms

III. Production of the Lymphokine Eosinophil Stimulation Promoter by Mouse T Lymphocytes^1,2,

From Vanderbilt University Medical School, Departments of Medicine and Microbiology and the Veterans Administration Hospital, Nashville, Tennessee 37203

Abstract

The lymphoid cell population responsible for production of eosinophil stimulation promoter (ESP), a lymphokine which increases migration of eosinophils, was investigated in murine Schistosoma mansoni infection. Con A challenge induced ESP production, whereas LPS did not. Prior treatment with anti-_C3H alloantiserum plus complement in vitro eliminated ESP production; in vivo treatment with rabbit anti-mouse thymocyte serum consistently reduced ESP production by splenic lymphoid cells, but affected lymph node cell ESP production only after exceptionally large doses. Thymocytes did not produce significant amounts of ESP; nor did lymphoid cells from congenitally athymic mice. Depletion of B lymphocytes and macrophages by nylon fiber adherence eliminated antigen-induced ESP production; this was partially restored by addition of non-immune, 72-hr peritoneal exudate cells. Con A-induced ESP production was not affected by nylon fiber treatment. These results demonstrate that ESP is produced by an ATS-sensitive, peripheralized T lymphocyte population, and suggest a macrophage requirement for antigen-induced production of this lymphokine.

Footnotes

¹ This work was supported by the Veterans Administration and National Institutes of Health Grant AI-11289 in participation with the U. S.-Japan Cooperative Medical Science Program.

² Portions of this study were presented at the 59th Annual Meeting of the American Association of Immunologists in Atlantic City, April, 1975.