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Cytotoxic Activity of Lymphocytes

VI. Heterogeneity of Cytotoxins in Supernatants of Mitogen-Activated Lymphocytes¹

From the Department of Surgery, Stanford University School of Medicine, Stanford, California 94305

Abstract

Two different lymphotoxins synthesized by human blood lymphocytes stimulated with phytohemagglutin (PHA) are described. The two toxins are called -LT and -LT relative to their elution order on gel chromatography. Their m.w. are 75,000 and 45,000 daltons, respectively. Both toxins appear as early as 7 hr after the addition of PHA, with the amount of -toxin exceeding that of -LT initially. Both toxins are differentiated from a third toxin (adherent cell toxin, ACT) made by plastic-adherent cells without requiring mitogen-stimulation. Depletion of macrophages or B cells does not affect the synthesis of either lymphotoxin. Monospecific antisera were elicited to -LT. Antisera elicited to -LT also neutralized -LT but to a significantly lesser degree. -LT appears to be the lymphotoxin described by most other workers. -LT is unstable at 37°C which may explain why the low m.w. lymphotoxin has not been described previously.

Footnotes

¹ This work was supported by Grants 5 R01-AM-12458-08 and 5 R01-CA-14777-02 from the National Institutes of Health and funds from the Beta Sigma Phi Dialysis and Transplant Fund.

² Present address: Department of Immunopathology, Scripps Clinic and Research Foundation, La Jolla, California 92037.

³ Reprint requests should be sent to: Zoltan J. Lucas, M.D., Department of Surgery, Stanford University Medical Center, Stanford, California 94305.