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Tumor-Associated Blocking Factors: Isolation from Sera of Tumor-Bearing Mice¹

From the Division of Immunology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 and the Departments of Microbiology and Immunology, Biochemistry, and Pathology, University of Washington School of Medicine, Seattle, Washington 98195

Abstract

An isolation and partial purification of tumor-associated blocking factors from the sera of tumor-bearing mice is described. Columns for affinity chromatography were prepared by coupling syngeneic tumor-immune antibodies to Sepharose 4B. Passage of serum through such immunoadsorbent columns removed all blocking activity from tumorbearers' sera; subsequent elution of the absorbent with 3 M NaSCN recovered the activity. The blocking material was further purified on Sephadex G-200. The data provide evidence for the presence of antigen in tumor-associated blocking factors and are compatible with the hypothesis that blocking factors often consist of antigen and antibodies in the form of immune complexes.

Footnotes

¹ This study was supported by Grants CA10188 and CA10189 from the National Cancer Institute, by Grant 1M-43F from the American Cancer Society, by Contract NIH-NCI-33372 within the Virus Cancer Program of the National Institutes of Health and by contract NO1CB 23887 from the National Cancer Institute.

² J. Tamerius was supported in part as a Graduate Research Trainee (Ca-5040), National Cancer Institute.

³ J. Nepom was supported in part as a Medical Scientist Trainee (GM 02103), National Institute of Health.