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Tolerance and Contact Sensitivity to DNFB in Mice

V. Induction of Tolerance with DNP Compounds and with Free and Membrane-Associated DNFB¹

From the Division of Clinical Immunology, Department of Medicine and Microbiology, University of Colorado Medical School, Denver, Colorado 80220

Abstract

Immunologic unresponsiveness (tolerance) was induced in a mouse model of contact sensitization to DNFB. The ability to induce tolerance varied with the chemical reactivity of the tolerogen; DNFB was highly tolerogenic, DNBSO₃ was moderately tolerogenic, and DNP-lysine was not tolerogenic. Although DNFB is considered a highly reactive compound, tracer studies of injected DNFB* showed that it was rapidly excreted. Further studies were therefore done with DNFB attached to mouse erythrocytes. Tolerance to DNFB-RBC was highly specific in vivo; mice tolerant to DNFB showed normal reactivity to TNCB (picryl chloride). Cells of mice tolerant to DNFB-RBC were also unresponsive to DNBSO₃ in vitro. Tolerance to DNFB, DNBSO₃, and DNFB-RBC all required time to develop, suggesting that an active process was involved.

Footnotes

¹ This work was supported in part by United States Public Health Service Grant AI 12685.