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Role of Mercaptoethanol and Endotoxin in Stimulating B Lymphocyte Colony Formation in Vitro¹

From the Walter and Eliza Hall Institute of Medical Research, Melbourne, 3050, Victoria, Australia

Abstract

Mercaptoethanol is necessary to permit B lymphocyte colony formation in semi-solid agar cultures of cells from normal mouse lymphoid organs. Transfer studies on developing colonies showed that, in part, this was a direct action on B lymphocyte colony cells but evidence was produced that in the presence of mercaptoethanol lymphoid organ cells release a factor promoting colony growth.

Endotoxin strongly potentiated B lymphocyte colony formation in vitro by a direct action on colony cells but in the absence of mercaptoethanol did not allow cell survival or proliferation.

Footnotes

¹ This work was supported by the Carden Fellowship Fund of the Anti-Cancer Council of Victoria and the National Cancer Institute, Washington, Contract NOI-CB-33854.

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