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The Journal of Immunology, 1976, 116: 575-578.
Copyright © 1976 by The American Association of Immunologists, Inc.
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Transformation of Human Peripheral Lymphocytes by Galactose Oxidase1

James F. P. Dixon2, John W. Parker and Richard L. O'Brien

From the Mossman Laboratories of Experimental Pathology, Department of Pathology, University of Southern California School of Medicine, Los Angeles, California 90033

Abstract

Human peripheral lymphocytes can be transformed by treatment with galactose oxidase alone. Prior treatment with neuraminidase enhances this effect. The aldehyde blocking agents thiocarbohydrazide, hydroxylamine, dimedone, and sodium borohydride block transformation when they follow, but not when they precede, galactose oxidase treatment. Thus, as is the case for periodate-induced lymphocyte transformation, the formation of free aldehyde at the cell surface would seem to be a critical event in the triggering of transformation by this agent.

The degree of transformation is highly variable from individual to individual, and also for the same donor at different times. However, the lymphocytes of some people give a consistently poor response to galactose oxidase. Similar results have been obtained for periodate-induced transformation of human lymphocytes, but to date this is unexplained.

Footnotes

1 This work was supported by Grants CA-14673 and CA-14089 from the National Cancer Institute, ACS-IDC 12 IN-21N. The Robert E. and May R. Wright Foundation. The Richard King Mellon Charitable Trusts, and The John A. Hartford Foundation, Inc.

2 Recipient of a Cancer Research Training Fellowship under National Cancer Institute Grant CA 05297.






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