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The Chemoattraction of Neutrophils by Heterologous and Homologous Cytotoxic Sera^1,2,

From the Department of Microbiology, University of Miami School of Medicine, Miami, Florida 33152

Abstract

Neutrophils (PMN) migrated in vitro chemotactically along concentration gradients of heterologous and homologous leukocytotoxic, complement-active sera. The heterologous systems consisted of normal, non-immune nurse shark or dog serum and human PMN; the homologous systems consisted of human or dog immune sera and human or dog PMN, respectively. Chemotaxis was induced 1) by fresh cytotoxic sera, 2) by complement alone if the cytotoxic antibodies were with the responding cells or if the cells were passively sensitized with the antibodies, and 3) by individual and combined complement components if the corresponding intermediate PMN-antibody-complement component complexes were the migrating cells. These observations represent a new mechanism of immune chemotaxis: the chemotactic stimulation is a direct consequence of a gradient-controlled immune reaction occurring at and involving the surface of the chemoattracted cell. This mechanism is distinctly different from that which depends on chemotactic factors produced or released independent of and at a distance from the responding cells.

Footnotes

¹ This work was supported by United States Public Health Service Grant AI 10726 from the National Institute of Allergy and Infectious Diseases and by institutional funds of the Veteran's Administration Hospital, Miami, Florida.

² In part based upon a dissertation submitted by D. W. to the Graduate School of the University of Miami as partial fulfillment of the requirements for the M.S. degree.

³ Send reprint requests to: J. A. Jensen, M.D., Department of Microbiology, School of Medicine, University of Miami, P. O. Box 520875, Miami, Florida 33152.