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Evidence for More Than One Ia Antigenic Specificity on Molecules Determined by the I-A Subregion of the Mouse Major Histocompatibility Complex

From the Immunology Branch, Division of Cancer Biology and Diagnosis, National Cancer Institute, Bethesda, Maryland 20014 and the Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan 48104

Abstract

Ia antigenic specificities determined by the I-A subregion of the mouse major histocompatibility complex have been examined in strain B10.D2 (H-2^d), C57BL/10 (H-2^b), and in a (C57BL/6xDBA/2) hybrid (BDF₁; H-2^b/d). Detergent solubilized, ³H-leucine-labeled antigen preparations were mixed with appropriate alloantisera and precipitation was induced either by addition of goat anti-mouse -globulin or by addition of protein A-bearing Staphylococci. Sequential precipitation analysis showed that in strain B10.D2, Ia specificities 8 and 11 were co-precipitable, and that in strain C57BL/10, Ia specificities 8 and 9 were co-precipitable. In contrast, precipitation of specificities 9 and 11 from a BDF₁ antigen preparation showed that these two Ia specificities were on separate molecules. The genetic implications of these data are discussed.

Footnotes

¹ Susan E. Cullen is the recipient of National Institutes of Health Research Service Award 1 F32CA5391-01 from the National Cancer Institute. Part of this work was supported by United States Public Health Service Grant AI 11962.

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