HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Glaser, M. Articles by Herberman, R. B. Search for Related Content PubMed Articles by Glaser, M. Articles by Herberman, R. B.

In Vitro Generation of Secondary Cell-Mediated Cytotoxic Response Against a Syngeneic Gross Virus-Induced Lymphoma in Rats

From the Laboratory of Immunodiagnosis, National Cancer Institute, Bethesda, Maryland 20014

Abstract

Cell-mediated cytotoxic responses against a syngeneic Gross virus-induced lymphoma, (C58NT)D, in W/Fu rats were generated in vitro by using mixed lymphocyte-tumor cell cultures. The source of responding cells was either spleens from normal rats or spleens from rats carrying or having rejected (C58NT)D tumors. Mitomycin C-treated (C58NT)D tumor cells were used as stimulating cells. The secondary anti-tumor cytotoxic response occurred more rapidly and reached higher levels than the primary response, and it was antigen specific. T cells, but not B cells or macrophages, were essential for both the induction and the effector phases of the secondary anti-tumor responses. These data suggest that specific memory T cells persist for long periods of time in the lymphoid organs of (C58NT)D immune rats, which can rapidly become cytotoxic upon re-exposure to antigen.

Footnotes

¹ Address reprint requests to: Moshe Glaser, Laboratory of Cell Biology, National Cancer Institute, Building 8, Room B-6, Bethesda, Maryland 20014.