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The in Vitro Suppression of Antigen- or Mitogen-Induced DNA Synthesis by Murine Spleen Cells after the Addition of Freshly Prepared Syngeneic Cells¹

From the Department of Microbiology, University of Illinois at the Medical Center, Chicago, Illinois, and the Division of Immunology, Hektoen Institute for Medical Research, Chicago, Illinois 60612

Abstract

The suppression of antigen- and mitogen-induced DNA synthesis by murine spleen cells in vitro was investigated. It appears that cultures receiving two signals exhibit marked suppression of DNA synthesis. The addition of KLH, PPD, Con A, PHA, or LPS to 72-hr-old cultures of KLH-stimulated murine spleen cells resulted in the suppression of DNA synthesis assayed at 144 hr. When small numbers of freshly prepared KLH-PPD SC or NSC were added to these cultures at 72 hr the suppression of DNA synthesis was abrogated. However, the addition of larger numbers of KLH-PPD SC or NSC resulted in increased suppression of DNA synthesis. Large numbers of KLH-PPD SC or NSC could substitute for the second stimulant (KLH, PPD, Con A, PHA, or LPS) in suppressing DNA synthesis. The addition of fresh cells obtained from KLH-PPD-immunized mice. Cells obtained 30 to 75 days post immunization were most effective in suppressing DNA synthesis.

Footnotes

¹ This research was supported by PHS-AI-09126. Presented in part at the 1975 FASEB meeting, Atlantic City, N. J.

² In partial fulfillment for the degree of Master of Science. Supported by PHS Training Grant AI-00335-08.

³ Reprint requests should be sent to Dr. Peter Baram, Division of Immunology, Hektoen Institute for Medical Research, 625 S. Wood St., Chicago, Illinois 60612