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A Microtiter Solid-Phase Radioimmunoassay for Hepatitis a Antigen and Antibody

From the Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, the Clinical Investigations Center, Naval Regional Medical Center, San Diego, California 92134, and The Children's Memorial Hospital, Chicago, Illinois

Abstract

A microtiter solid phase radioimmunoassay for hepatitis A antigen (HA Ag) and antibody (anti-HA) was developed. The test was more sensitive than immune adherence hemagglutination for detecting HA Ag and almost as sensitive for detecting anti-HA. The specificity and sensitivity of reagents were examined and optimum conditions for the test were determined. Radioimmunoassay, immune adherence hemagglutination, and immune electron microscopy were compared for detecting anti-HA. A serologic response to HA Ag was detected in paired sera from patients with type A hepatitis but not from patients with type B or non-A, non-B hepatitis by all three techniques.

Footnotes

¹ Send reprint requests to: Dr. Robert H. Purcell, Head, Hepatitis Virus Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Building 7, Room 202, Bethesda, Maryland 20014.

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