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Immunosuppressive Factor(s) Extracted from Lymphoid Cells of Nonresponder Mice Primed with L-Glutamic Acid⁶⁰-L-Alanine³⁰-L-Tyrosine¹⁰ (GAT)

I. Activity and Antigenic Specificity¹

From the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

Abstract

The synthetic terpolymer of L-glutamic acid⁶⁰-L-alanine³⁰-L-tyrosine¹⁰ (GAT) not only fails to elicit a GAT-specific antibody response in nonresponder mice, but also prior injection of GAT specifically decreases the ability of nonresponder mice to develop a GAT-specific antibody response to a subsequent challenge with GAT-MBSA. This inhibition is mediated by GAT-specific suppressor T cells. Further, a suppressive factor can be extracted from lymphoid cells of GAT-primed nonresponder mice that inhibits the development of primary GAT-specific antibody responses to GAT-MBSA and to GAT-PRBC by normal syngeneic mice. The suppressive activity is dose-dependent and absorbed by GAT-Sepharose, but not by BSA-Sepharose. The suppressive activity elutes from a G-100 Sephadex column in the same fraction as ovalbumin, suggesting its m.w. is approximately 45,000 daltons.

Footnotes

¹ This investigation was supported by United States Public Health Service Grants AI-09920 and AI-09897 from the National Institute of Allergy and Infectious Diseases.

² Recipient of United States Public Health Service Research Career Development Award 5K4-AI-70173 from the National Institute of Allergy and Infectious Disease.

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