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Cell-Mediated Immunity to Friend Virus-Induced Leukemia

II. Characteristics of Primary Cell-Mediated Cytotoxic Response¹

From the Laboratory of Immunodiagnosis, National Cancer Institute, Bethesda, Maryland 20014 and Litton-Bionetics, Inc., Kensington, Maryland 20795

Abstract

The primary cell-mediated cytotoxic response to a Friend virus-induced leukemia, FBL-3, in C57BL/6 mice was measured by the ¹²⁵IUdR release assay. Intraperitoneal (i.p.) inoculation of 1 x 10¹ FBL-3 cells produced progressive tumor growth (progressors); subcutaneous (s.c.) inoculation of as many as 5 x 10⁶ FBL-3 cells produced only transient tumor growth (regressors), and these mice would subsequently resist i.p. challenge of FBL-3 cells at 3 days after s.c. inoculation. The kinetics of the primary cell-mediated cytotoxic response of regressors was biphasic. Significant cytotoxicity could be detected at 3 to 5 days after s.c. inoculation of 5 x 10⁶ FBL-3 cells, peaked at days 10 to 14, declined to a very low level or became undetectable around days 20 to 30; then the reactivity reappeared and persisted at least up to 60 days. In progressors, the kinetics of the cell-mediated cytotoxic response was similar to the regressors, but the reactivity was much lower. The cytotoxic response was found to be T cell dependent, during both the first peak (days 10 to 14) and the second peak (days 40 to 60). In adoptive transfer experiments, lymphocytes from regressors gave 90% protection against i.p. challenge of FBL-3; lymphocytes from progressors only gave 40% protection.

Footnotes

¹ This work was supported in part by contract NIH-NCI-G-72-3878 with Litton Bionetics, Inc., 5516 Nicholson Lane, Kensington, Maryland 20795.

² Laboratory of Immunodiagnosis, National Cancer Institute, Bethesda, Maryland 20014.

³ Present address: Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland 20014. Please send correspondence to Dr. Chou-Chik Ting, National Cancer Institute, Bldg. 8, Room 219, Bethesda, Maryland 20014.

⁴ Litton-Bionetics, Inc., 5516 Nicholson Lane, Kensington, Maryland 20795.

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