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Antigenic Determinants of Adenovirus Capsids

I. Measurement of Antibody Cross-Reactivity

From the Divisions of Immunology and Virology, National Institute for Medical Research, Mill Hill, London, NW7, 1AA, England

Abstract

Evidence is presented that the type-specific antibody to the adenovirus hexon is not simply the antibody with the highest avidity for cross-reactive determinants, but is a distinct, minority population that recognizes separate determinants. To quantify it, we have developed an inhibition method with radio-immunoprecipitation (RIP) as a sensitive assay for the type-specific antibody that remains after all the excess of cross-reactive antibody has been blocked by heterologous antigen.

During the primary response, 0.1 to 1% of antibody to types 2 or 5 hexon is type-specific, but after boosting, this population may reach 10 to 20%. Antibody to fiber is more than 70% type-specific during primary and secondary responses. The cross-reacting antibody can be removed on immunoabsorbent columns without affecting the virus neutralization titer of the serum.

Footnotes

¹ Present address: Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.

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