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A Properdin System Intermediate Formed by Zymosan and Serum at 0°C¹

From the Department of Medical Immunology, The Public Health Research Institute of the City of New York Inc., New York, New York 10016

Abstract

The interaction of guinea pig or rat serum with Z at 0°C leads to the formation of a properdin pathway intermediate, ZPI, whose activity is assessed by its capacity to deplete the titer of cobra venom inducible lysis in diluted rat serum. The formation of ZPI does not require C1 or C2 and is not diminished by prior absorption of the serum with Z. In contrast, removal of P suppresses ZPI formation. Both Ca⁺⁺ and Mg⁺⁺ are essential cofactors for the formation of this intermediate whose function is abrogated in the presence of anti-C3, anti-P, but not by anti-Factor B. Since C4-deficient guinea pig serum is also effective in ZPI formation, the data suggest that ZPI is an alternative pathway intermediate containing both P and C3.

Footnotes

¹ This study was supported in part by the National Science Foundation, Grant GB-31738X; The American Cancer Society, Inc., Grant IC-391; and United States Public Health Service, Grant A108710.