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From the Departments of Laboratory Medicine and Pathology, Pediatrics, and Surgery, University of Minnesota, Minneapolis, Minnesota 55455
Abstract
A variety of methods have been devised for the study of spontaneous and directed cell migration. Among these, the membrane filter method introduced by Boyden in 1962, with its more recent modifications, has become the technique of choice for studies of leukocyte migration in vitro. This method, however, cannot be applied without alteration to studies of chemotaxis and spontaneous migration of cells of different types. We describe in this report a new and simple method for studying human leukocyte chemotaxis, in vitro, which is based upon migration of cells under agarose gel. This method has application to both polymorphonuclear leukocytes and monocytes, permits measurement of both chemotaxis and spontaneous migration, requires fewer cells per test, and is rapid, simple, reproducible, and inexpensive to set up.
Footnotes
1 This study was supported by United States Public Health Service Grants A1 12402 and CA 11605, National Cancer Institute Contract NO1-CB 43948, and grants from the Kidney Foundation of the Upper Midwest, Minnesota Heart Association, and University of Minnesota Graduate School.
2 Address reprint requests to Dr. Nelson, Box 185, Mayo Memorial Building, University of Minnesota Hospitals, Minneapolis, Minn. 55455.
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