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Response of Mouse Splenic Lymphocytes to Timothy Pollen Antigens in a Microculture System¹

From the Oregon Regional Primate Research Center, Beaverton, Oregon 97005

Abstract

Spleen cells from LAF₁ mice were stimulated in a microculture system with T and B cell mitogens or antigens of timothy pollen. Only cells from mice immunized with crude timothy pollen extract (WST) or a major antigen of timothy pollen conjugated to Ascaris (antigen B-Ascaris) responded to timothy antigens in vitro. Optimum responses were obtained at 120 to 144 hr of culture with 5 to 10 µg WST per culture and ranged from three to 10 times greater than cell background. No correlations could be found between the optimum antigen concentration or the maximum response and the immune status of the spleen cell donor. Response could be inhibited by a dialyzáble fraction of timothy pollen, antigen D, which is a monovalent form of a major antigen of timothy pollen.

Footnotes

¹ This work was supported by United States Public Health Service Grants AI-06274 and AI-09232-04, and was submitted in partial fulfillment for the Ph.D. requirement at the University of Oregon Medical School, Portland, Oregon.