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Cell-Mediated Immune Responses in Vitro

III. Elimination of Specific Cytotoxic Lymphocyte Responses by ³H-Thymidine Suicude¹

From the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115

Abstract

The role of cellular proliferation in the development of cytotoxic lymphocyte (CL) responses in one-way mixed lymphocyte reactions was investigated by using tritiated thymidine of high specific activity to kill proliferating cells. To develop maximum CL responses, responding lymphoid cells must proliferate for approximately 72 hr; thereafter, precursors of CL appear to differentiate into active CL without further proliferation. Different alloantigen-sensitive precursor cell populations participate in the CL responses to each of two sets of stimulating alloantigens. When cells responding to one set of alloantigens were selectively destroyed after incorporating the hot thymidine, the surviving cells retained the capacity to develop a normal CL response to the second set of alloantigens.

Footnotes

¹ This investigation was supported by United States Public Health Service Program Project Grant CA-14723 from the National Cancer Institute.

² Recipient of U. S. Public Health Service Postdoctoral Fellowship CA-00566 from the National Cancer Institute.

³ Recipient of U. S. Public Health Service Research Career Development Award 5K4-AI-70173 from the National Institute of Allergy and Infectious Diseases.