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Immunologic Properties of Mast Cells from Rats Infected with Nippostrongylus Brasiliensis¹

From the Department of Medicine, The Johns Hopkins University School of Medicine, Good Samaritan Hospital, Baltimore, Maryland 21239

Abstract

The concentration of IgE in the serum of Sprague-Dawley rats increased after infection with Nippostrongylus brasiliensis (NB). The IgE concentration in normal rats was less than 1 µg/ml. After re-infection with NB, the concentration increased to 100 to 300 µg/ml. Mast cells were purified from peritoneal cells of both normal and NB-infected animals. Purified mast cells from the infected animals released histamine upon exposure to NB antigen. The antibody specific for IgE released histamine from purified mast cells of both normal and infected animals. Dose-response curves of histamine release suggested that mast cells from NB-infected animals bear more IgE molecules than normal mast cells. Binding of ¹²⁵I-labeled rat E myeloma protein with normal mast cells was demonstrated by autoradiography. Under the same experimental conditions, mast cells of infected animals were not labeled with ¹²⁵I-IgE. Mast cells from both normal and infected animals failed to combine ¹²⁵I-labeled IgG. The number of IgE molecules bound per mast cell was determined by incubating ¹²⁵I-labeled IgE with purified mast cells. When mast cells were incubated in 0.6 to 2 µg/ml of IgE, the number of IgE molecules combined with the mast cells from infected animals was about 10% of that bound with normal mast cells. The results indicated that a large proportion of IgE receptors on mast cells of infected animals was occupied by their own IgE. No significant difference was observed between normal mast cells and those of infected animals with respect to histamine content and intracellular levels of cyclic nucleotides.

Footnotes

¹ This work was supported by Research Grant AI-10060 from the United States Public Health Service and a grant from Lillia Babbit Hyde Foundation. This paper is publication No. 177 from the O'Neill Laboratories, the Good Samaritan Hospital.

² Recipient of a grant of the Deutsche Forschungsgemeinschaft Bad Godesberg, Kö 427/2.