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The Effect of Thiols on Immunologic Release of Slow Reacting Substance of Anaphylaxis

I. Human Lung¹

From the Department of Immunology, Research Institute of the Hospital for Sick Children at the University of Toronto, Toronto, Ontario, Canada, M5G 1X8

Abstract

Preincubation of human lung fragments with cysteine for 2.5 to 5.0 min resulted in dose-dependent, selective enhancement of the antigen-induced or anti-IgE-induced formation and release of slow reacting substance of anaphylaxis (SRS-A). Comparable effects were observed with sodium sulfide and thioglycolate but not with other more potent reducing agents or metabolites of cysteine. Sulfhydryl alkylated derivatives of cysteine were ineffective. The effects observed with the active thiols were easily reversed and could not be attributed to an action in the bioassay or on SRS-A itself. The physicochemical characteristics of the contractile activity were identical to those described for SRS-A.

Footnotes

¹ Supported by Grant MT-4605 from the Medical Research Council of Canada.