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In Vitro Human Reactivity to Staphylococcal Phage Lysate¹

From the Department of Immunology, Litton Bionetics, Inc., Kensington, Md. 20795, and the Medical Center, Keesler Air Force Base, Biloxi, Miss. 39531, and the Laboratory of Immunodiagnosis, National Cancer Institute, Bethesda, Md. 20014

Abstract

The cell-mediated reactivity of normal individuals to staphylococcal phage lysate (SPL) were tested in vitro in the lymphocyte stimulation (LS) and leukocyte migration inhibition (LMI) assays. There were 95% positive responses in LS (stimulation ratio 3 with p < 0.01) and 67% positive responses in LMI (migration index 0.80). Enriched subpopulations of T and B lymphocytes were prepared with rosette formation and density gradient centrifugation. SPL stimulated lymphoproliferative responses in both T and B cell subpopulations whereas phytohemagglutinin (PHA) stimulated only the T cell subpopulation. Cord blood leukocytes were tested in the LS assay and 41% gave positive responses to SPL, 81% to PHA, and 17% with SLO. SPL appears to be a useful reagent for the in vitro study of cell-mediated reactivity, and may provide somewhat different information from that obtained with other mitogens or antigens.

Footnotes

¹ This work was supported in part by United States Public Health Service Contracts NIH-NCI-72-3227 and NIH-NIDR-7-72-14R from the National Institutes of Health, Bethesda, Md. 20014.