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Modulation of HL-A Antigens by Anti-HLA Antiserum: Effects on the Cytotoxicity Assay and Mixed Leukocyte Reaction¹

From the Division of Allergy and Immunology and Department of Pathology, School of Medicine, University of Pennsylvania and VA Hospital, Philadelphia, Pennsylvania 19174

Abstract

Modulation of human leukocyte antigen (HLA) was attempted by treating leukocytes with specific anti-HLA antiserum or by their passage through columns coated with anti-HLA or a double layer of HLA-anti-HLA. The modulated cells were resistant to the cytotoxic effects of the anti-HLA, and they were poor stimulators and good responders to allogeneic cells in the unidirectional mixed leukocyte reaction. Modulated cells regained their HLA 16 hr after modulation if kept in cell suspension alone. The proliferative responses of modulated cells to mitogens were as good as non-modulated cells, indicating that modulation was probably not caused by depletion of lymphoid cells. Supernatants of modulated cells that were incubated over-night or preformed HLA-anti-HLA complexes were capable of suppressing and enhancing the MLR of specific cells depending on the dose used. The similarities of modulation of HLA to other lymphocyte receptors and the limitation of application of the modulation phenomenon to transplantation of allogeneic cells are discussed.

Footnotes

¹ This work was supported by Veterans Administration Grant 642-0030 and United States Public Health Service-Allergy Immunology Grant 00319.

² Present address: Department of Pathology, Cantonal Hospital, Lausanne, Switzerland.

³ Post-doctoral Fellow, Medical Research Council of Canada.

⁴ Address reprint requests to: Dr. N. I. Abdou, University of Kansas Medical Center, Allergy and Immunology Section, 39 and Rainbow Boulevard, Kansas City, Kansas 66103.