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Class, Amounts and Affinities of Anti-Dinitrophenyl Antibodies in Chickens

I. Production of 7S and 17S Antibodies of Equal Affinity by Intravenous Injection of Antigen¹

From the Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822

Abstract

Repeated intravenous injections of maximally coupled dinitrophenylated bovine -globulin elicited both 7S and 17S anti-dinitrophenyl antibodies in chickens. Only antibodies of low affinity were produced regardless of the priming dose, the interval between injections, and the number of injections. The 7S and 17S antibodies isolated from individual animals had identical affinities and heterogeneity indices. The concentrations of antibodies formed were uniformly low despite many injections over a prolonged period. These studies indicate that stimulation by antigen alone may not be sufficient for the induction of predominant 7S antibody formation and for the synthesis of high affinity antibody.

Footnotes

¹ This investigation was supported by National Institutes of Health Research Grant Number AI-05660 from the National Institutes of Allergy and Infectious Diseases.

² Present Address: Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado.

³ Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy, University of Hawaii, Honolulu.