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The Identification of HL-A Antigens on Fresh and Cultured Human Endothelial Cells¹

From the Departments of Medicine and Pathology, The New York Hospital-Cornell Medical Center, and The Tissue Typing Laboratory of The Division of Serology and Genetics, The New York Blood Center, New York, New York, 10021

Abstract

Human endothelial cells were obtained from the umbilical cord veins of 16 newborns by methods previously described and tested for HL-A antigens by a microcytotoxicity method. HL-A antigens were present on all endothelial cell lines tested. When the HL-A phenotypes of fresh endothelial cells and autologous fetal lymphocytes were compared, a concordance of 70% was observed. When the HL-A phenotypes of maternal lymphocytes and fresh endothelial cells were compared, a maternal contribution to the endothelial cell phenotype was evident in 72% of the possible common antigens. Some HL-A antigens were deleted from 11 of 16 endothelial cell lines that were re-typed after 2 weeks in tissue culture. The majority (90%) of deleted antigens were from the second HL-A locus. When three lines of endothelials cells were again re-typed after 6 weeks in culture, no further changes in antigenicity were noted. These findings: a) demonstrate that HL-A antigens are present on human endothelium and suggest that endothelial cells are actively involved in establishing the immunogenicity of a graft, and b) demonstrate that the HL-A antigens on human endothelial cells may be modulated by in vitro culture.

Footnotes

¹ This work was supported by National Institutes of Health Grants HL-14810, HE-01803, HL-09011, American Heart Association Grant AHA-72-900 and Grants from the Arnold R. Krakower Hematology Foundation and the Cross Foundation.

² A. G. is a Jonas Salk Scholar of the City University of New York.

³ E. A. J. is a Career Scientist of the Health Research Council of the City of New York (I-808).

⁴ To whom reprint requests should be sent.