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Humoral Immunostimulation

III. Requirements for Divalent Antibody and Cellular Aggregation¹

From the Departments of Pediatrics and Medicine, Washington University School of Medicine, St. Louis, Misouri 63110

Abstract

The intracellular incorporation of radioactive nucleoside into mouse L cells was stimulated by rabbit anti-L cell IgG antibody and by its divalent pepsin F(ab')₂ fragment but not by univalent pepsin Fab' or papain Fab fragments. Divalent antibody also caused the cells to grow in an aggregated pattern and undergo capping, but univalent antibody did not. Prevention of aggregation of antibody-treated cells by growth in agar abrogated immunostimulation. Univalent antibody fragments partially blocked stimulation of cells by divalent antibody and binding of ¹²⁵I-labeled divalent antibody to cells. These results suggest that immunostimulation of cell growth is dependent upon divalent antibody and that aggregation of cells is an associated event.

Footnotes

¹ This investigation was supported by a United States Public Health Special Research Fellowship (IF03-AI53856) from the National Institute of Allergy and Infectious Diseases (WTS), by a Research Scholar Award of the Cystic Fibrosis Foundation (WTS), by Grant 5RO1 CA 12626 from the National Institutes of Health, and by a grant from the American Cancer Society (IM-54).