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The Expression of -Like Antigen by Rat Peripheral Lymphocytes: Serologic and Functional Studies¹

From the Division of Biology, California Institute of Technology, Pasadena, California 91109

Abstract

AKR/Cum mice (Thy-1^b = ^C3H) immunized with nucleated cells from WF rat thymus, Peyer's patches, peritoneal exudate, mesenteric lymph nodes, blood, bone marrow, or spleen produced antibodies cytotoxic for AKR/J (Thy-1^a = ^AKR) but not for AKR/Cum thymocytes. The specificity of these antibodies for the Thy-1.1 (-AKR) antigen was confirmed by tests using thymocytes from backcross mice segregating at the Thy-1 locus. This result suggested that the rat lymphocyte antigen cross-reactive with Thy-1.1 was expressed by at least some members of each of the rat lymphoid cell populations tested. AKR/Cum mice immunized with killed rat cells also produced anti-Thy-1.1 antibodies; thus indicating that further differentiation of the injected cells was not a prerequisite for the anti-Thy-1.1 response.

Unexpectedly, about 9% of unimmunized adult AKR/Cum males were found to be producing antibodies against Thy-1.1. To our knowledge, natural antibodies of this specificity have not been previously reported.

Finally, it was found that peritoneal exudate cells taken from WF rats previously immunized with EL4 mouse leukemia cells were neither killed nor functionally inactivated by treatment with anti-Thy-1.1 antibodies and complement.

Footnotes

¹ This work was supported by United States Atomic Energy Commission Contract AT 04-3-767 and United States Public Health Service Grant GM 00086.

² Present address: Institute for Immunology, 487 Grenzacherstrasse, CH-4058 Basel, Switzerland.