HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Thomas, D. W. Articles by Talmage, D. W. Search for Related Content PubMed Articles by Thomas, D. W. Articles by Talmage, D. W.

Regulation of the Immune Response: Production of a Soluble Suppressor by Immune Spleen Cells in Vitro¹

From the Department of Microbiology, University of Colorado Medical School, Denver, Colorado 80220

Abstract

The addition of ovalbumin-immune spleen cells (Ova ISC) to sheep erythrocyte-immune spleen cells (SRBC ISC) in Mishell-Dutton-type cultures resulted in a dramatic reduction of PFC to SRBC and was dependent upon the addition of soluble Ova at low concentrations of Ova ISC. The suppressing cells in the Ova ISC were shown to be irradiation sensitive, depleted by anti- antiserum and complement treatment, and did not absorb to glass bead columns. Ova ISC-induced inhibition also occurred in culture chambers across a cell-impermeable membrane and a soluble inhibitor was recovered in chambers opposite the Ova ISC. This suppressor factor was sensitive to trypsin treatment and to heating at 80°C, but not to 70°C, for 30 min. The molecular weight, as determined by sucrose gradient analysis, was between 55,000 and 60,000 daltons. This suppressor factor appears to be distinct from a T-cell "helper" factor which was found to be sensitive to heating at 70°C for 30 min. We propose that this suppressor factor participates in the termination of most immunologic responses and is responsible for the antigenic competition phenomenon.

Footnotes

¹ This work was supported by National Institutes of Health Grants AI-03047, CA-10641, and 5 T01 GM02219.