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IgG Diphtheria Antitoxin Responses from Human Tonsil Lymphocytes Induced by Anti--Chain Antibodies¹

From the Department of Medicine, The Johns Hopkins University School of Medicine at the Good Samaritan Hospital, Baltimore, Maryland 21239

Abstract

Human tonsil lymphocytes were cultured for 24 hr with purified antibodies specific for human IgG heavy chain determinants (anti--chain) and then cultured for 6 days in Marbrook tubes. IgG diphtheria antitoxin was measured in the culture supernatants by antigen-binding radioimmunoassay. In cultures from eight tonsils, anti--chain stimulated significant IgG antitoxin formation; in each case parallel cultures stimulated with toxoid gave good responses. The specificity of the antibody produced was established by showing that supernatants from cultures stimulated with anti--chain will neutralize toxin. Total IgG in culture supernatants was measured by radioimmunoassay and it was found that cultures stimulated with anti--chain generally produced less total IgG than unstimulated cultures. In time course experiments IgG antitoxin formation increased rapidly after the 3rd day whereas over 50% of the total IgG was produced in the first 3 days. Evidence is presented which suggests that anti--chain acts differently on different groups of B cells.

Footnotes

¹ This work was supported by Research Grant 11202 from the United States Public Health Service. This is publication No. 162 from the O'Neill Laboratories of the Good Samaritan Hospital.

² Postdoctoral trainee supported by Training Grant T 01-AI-00423 from the National Institutes of Health. Present address: Clinical Research Centre, Watford Road, Harrow, Middlesex HAI 3UJ, England.