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Enhancement and Hyperacute Rejection of Skin Grafts in the Mouse

Failure to Separate Cytotoxic and Enhancing Antibodies from Alloantiserum with Physicochemical Methods¹

From the Department of Medicine, Division of Nephrology, University of Nijmegen, Sint Radboudziekenhuis, Nijmegen, the Netherlands

Abstract

B6AF1 anti-B10.D2 ascites fluid was fractionated by molecular sieving and cation exchange chromatography in an attempt to separate cytotoxic and enhancing antibodies. Five fractions were obtained which showed no overlap on analysis with isoelectric focusing. Despite this complete physicochemical separation all fractions contained detectable amounts of 7S IgG1 and 7S IgG2. Furthermore, enhancement of B10.D2 skin grafts in B6AF1 recipients could be induced with all the fractions. Cytotoxic activity was also present in the fractions. This was not only shown in vitro by the cytolysis of B10.D2 lymphoid cells in the presence of rabbit complement, but also by the ability of the different fractions to induce hyperacute destruction of B10.D2 skin grafted onto B6AF1 mice after i.v. injection together with rabbit complement. Thus, we were unable to separate the alloantiserum in cytotoxic and enhancing fractions by physicochemical means.

Footnotes

¹ This study was supported by grants from the Netherlands Foundation for Medical Research (FUNGO) and from the Netherlands Kidney Foundation.